“Mathematical models and estimates support that the use of non-Bt refuges would render Bt crops effective for more than 20 years,” says institute entomology researcher Juan Luis Jurat-Fuentes.

“However, we’re starting to see the first cases of field-evolved resistance, suggesting that some of the high-dose and refuge requirements have not been fulfilled. Companies are now developing new Bt crops containing different combinations of Bt toxins to reduce dependency on refuges and further delay resistance while providing better control of the insects.”

Through collaborators at the USDA, Jurat-Fuentes acquired Bt-resistant fall armyworm caterpillars from Puerto Rico to study. In the lab, he and students Siva Jakka and Liang Gong are working to pin-point the exact mechanism responsible for resistance to Bt corn.

That knowledge would enable agrochemical companies to develop improved Bt crops and sensitive assays to quickly determine if resistant insects are present in production fields.

While the EPA currently mandates such monitoring, the methods used require capturing insects and analyzing subsequent generations in the lab, which is lengthy, costly and laborious.

“It takes months to do this and a lot of work,” Jurat-Fuentes says. “So if we come up with a DNA-based assay that can be done in a day with a caterpillar or moth from the field and tell whether the resistance gene is present, then we can have a great economic impact on monitoring for resistance.

We do have collaborations with some companies that support part of our research, so what we find is of great scientific and applied interest.”

Jurat-Fuentes’ research is also funded by a grant from the USDA National Institute of Food and Agriculture, highlighting its importance. For the promise of his research, Jurat-Fuentes was one of 18 researchers worldwide last year who was honored as a DuPont Young Professor.

“To develop resistance, an insect must change something,” Jurat-Fuentes explains. “Although we have some ideas from lab research, our project represents the first effort to understand what field insects change to become resistant to Bt crops. We are also interested in learning about how resistance is transmitted and the fitness of the resistant compared to susceptible armyworms to evaluate and improve current resistance management mandates.

“If we can delay emergence of resistance through better detection and control of the resistant insects, we can perhaps preserve the effectiveness of Bt for years to come.”

UT AgResearch operates 10 unique research facilities across the state of Tennessee. In addition to its agricultural research programs, the UT Institute of Agriculture also provides instruction, research and public service through the UT College of Agricultural Sciences and Natural Resources, the UT College of Veterinary Medicine, and UT Extension offices in every county in the state.